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We observed the slight decrease in the expressions of MEK1/2 and ERK1/2 in AsPC-1 cells upon treatment with Amatuximab, but not in significant

We observed the slight decrease in the expressions of MEK1/2 and ERK1/2 in AsPC-1 cells upon treatment with Amatuximab, but not in significant. the blot using the images in those the blotting picture and marker were merged. 12885_2020_7722_MOESM3_ESM.pdf (46K) GUID:?74064588-6D0F-453E-B6B7-931D6E5023B7 Additional file 4: Supplemental Figure2. Corresponding uncropped full-length blot images for Fig. ?Fig.1a.1a. The cropped blots were marked with black frame. Densitometric analysis of western blots was performed using a ChemiDoc XRS Plus system with Image Lab Software (Bio-Rad, Hercules, CA, USA). We cut the membranes according to the standard protein size markers and detected the blot using the images in those the blotting picture and marker were merged. 12885_2020_7722_MOESM4_ESM.pdf (148K) GUID:?217EEED3-2D2F-480D-984B-72A7AA053301 Additional file 5: Supplemental Figure?3. Corresponding uncropped full-length blot images for Fig. ?Fig.3a.3a. The cropped blots were marked with black frame. Densitometric analysis of western blots was performed using a ChemiDoc XRS Plus system with Image Lab BRM/BRG1 ATP Inhibitor-1 Software (Bio-Rad, Hercules, CA, USA). We cut the membranes according to the standard protein size markers and detected the blot using the images in those the blotting picture and marker were merged. 12885_2020_7722_MOESM5_ESM.pdf (408K) GUID:?BDE82736-9CB4-45CF-9063-B273B0212E6A Additional file 6: Supplemental Figure?4. Corresponding uncropped full-length blot images for Fig. ?Fig.3b.3b. The cropped blots were marked with black frame. Densitometric analysis of western blots was performed using a ChemiDoc XRS Plus system with Image Lab Software (Bio-Rad, Hercules, CA, USA). We cut the membranes according to the standard protein size markers and detected the blot using the BRM/BRG1 ATP Inhibitor-1 images in BRM/BRG1 ATP Inhibitor-1 those the blotting picture and marker were merged. 12885_2020_7722_MOESM6_ESM.pdf (563K) GUID:?2139F27D-6E32-42E5-A5AE-9BD0F80DD3B2 Additional file 7: Supplemental Figure?5. Corresponding uncropped full-length blot images for Fig. ?Fig.3c.3c. The cropped blots were marked with black frame. Densitometric analysis of western blots was performed using a ChemiDoc XRS Plus system with Image Lab Software (Bio-Rad, Hercules, CA, USA). We BRM/BRG1 ATP Inhibitor-1 cut the membranes according to the standard protein size markers and detected the blot using the images in those the blotting picture and marker were merged. 12885_2020_7722_MOESM7_ESM.pdf (359K) GUID:?DF665029-0D62-4C6D-A077-607B4292C68C Additional file 8: Supplemental Figure?6. Corresponding uncropped full-length blot images for supplemental Figure 1. The cropped blots were marked with black frame. Densitometric analysis of western blots was performed using a ChemiDoc XRS Plus system with Image Lab Software (Bio-Rad, Hercules, CA, USA). We cut the membranes according to the standard protein size markers and detected the blot using the images in those the blotting picture and marker were merged. 12885_2020_7722_MOESM8_ESM.pdf (166K) GUID:?5697AD65-A4D2-4EBE-8AC1-2F23008D723F Additional file 9: Supplemental Figure?7. Analysis of mesothelin expression in the four human pancreatic cancer cells by immunocytochemistry: (a) AsPC-1, (b) Capan-2, (c) Panc-1 and (d) MIA Paca-2 cells. The image of Capan-2 was taken by deferent researcher in another time, so in a little bit deferent condition. Scale bar, 100?m. 12885_2020_7722_MOESM9_ESM.pdf (134K) GUID:?77230E80-07B5-4C2A-B519-17282E8427F2 Data Availability StatementAll data generated or analysed during this study are included in this published article and its supplementary information files and available. Abstract Background Mesothelin is a 40-kDa glycoprotein that is highly Rabbit polyclonal to DCP2 overexpressed in various types of cancers, however molecular mechanism of mesothelin has not been well-known. Amatuximab is a chimeric monoclonal IgG1/k antibody targeting mesothelin. We recently demonstrated that the combine therapy of Amatuximab and gemcitabine was effective for peritonitis of pancreatic cancer in mouse model. Methods We discover the role and potential mechanism of mesothelin blockage by Amatuximab in human pancreatic cells both expressing high or low level of mesothelin in vitro experiment and peritonitis mouse model of pancreatic cancer. Results Mesothelin blockage by Amatuximab lead to suppression of invasiveness and migration capacity in AsPC-1 and Capan-2 (high mesothelin expression) and reduce levels of pMET expression. The combination of Amatuximab and gemcitabine suppressed proliferation of AsPC-1 and Capan-2 more strongly than gemcitabine alone. These phenomena were not observed in Panc-1 and MIA Paca-2 (Mesothelin low expression). We previously demonstrated that Amatuximab reduced the peritoneal mass in mouse AsPC-1 peritonitis model and induced sherbet-like cancer cell aggregates, which were vanished by gemcitabine. In this study, we showed that the cancer stem cell related molecule such as ALDH1, CD44, c-MET, as well as proliferation related molecules, were suppressed in sherbet-like aggregates, but once sherbet-like aggregates attached to peritoneum, they.