Na?ve CD8+ T cells were isolated from PBMCs based on a two-step process using specific microbeads (Miltenyi Biotec). T cells. Spots count for na?ve CD4+ T cells specific to nickel from donors PR4, PR5, PR6, PR12, PR13, PR15, PR19, PR20, PR29, and PR43. Purified CD4+ T cells from each donor were seeded in multiple wells […]