Scopolamine (0
Scopolamine (0.4 mg Kg-1, i. organizations (n=5 each) comprising Group A; ovary undamaged adult rats, Group B; ovariectomized (ovx) adult rats, Group C; E2 treated ovx adult rats, Group D; ovary undamaged aged rats, Group E; ovx aged rats, and Group F; E2 treated ovx aged rats (pets of Group D, E and F had been kept for ageing until 1 . 5 years). Evaluation of results demonstrated how the ovariectomy led to deterioration 7,8-Dihydroxyflavone of hippocampal cytoarchitecture and improved apoptosis as well as the ER alpha and beta immunoreactive (ir) neurons had been significantly (in air blood sugar deprivation-pc-12 cell style of cerebral heart stroke Singh G1, Siddiqui MA1, Kashyap MP1, Pant KK2, Gupta YK3, Pant Abdominal1 1Indian Institute of Toxicology Study, Lucknow; 2CSM Medical College or university, Lucknow; 3All India Institute of Medical Sciences, New Delhi, India. Oxidative tension mediated neuronal damage is well recorded in ischemic cerebral heart stroke. The amounts of antioxidants have already been looked at for his or her neuroprotective potential and it is one of these. However, the systems involved in restorative treatment of in cerebral heart stroke are however to explore. Therefore, attempts had been initiated to recognize the factors of restorative interventions of using oxygen-glucose deprivation (OGD)-Personal computer-12 cell style of ischemic heart stroke. The model was made by revealing the Personal computer-12 cells to OGD insult for 6 h carrying out a re-oxygenation of 24 h under normoxia condition in glucose including medium. Biologically secure concentrations (25, 50, 100 g/ml) of determined through MTT and LDH assays had been utilized. Three treatment schedules had been designed viz., pretreatment group (treatment for 24 h prior to OGD insult), post treatment group (treatment for 24 h after OGD insult) and whole treatment group (treatment starting from 24 h prior to OGD insult and continued till the completion of re-oxygenation period). Parallel units without OGD insult, OGD insult without treatment also run under identical 7,8-Dihydroxyflavone conditions and served as basal and OGD settings respectively. Following respective treatments, cells were analyzed to study the mediated repair of oxidative stress induced alterations viz., reactive oxygenation varieties (ROS), nitric oxide (NO), lipid peroxidation (LPO), glutathione content material (GSH), membrane potential, dopamine receptor (DA-D2) and prostaglandin E2 (PGE2). In general, a dose dependent significant recovery in the levels of PGE2, GSH and NO could be recorded in all three treatment organizations, however, pre- and post-treatment organizations were found to be most effective on ROS and DA-D2 repair. No significant response could be recognized for LPO and membrane potential. The preliminary getting suggests the restorative treatment of in cerebral stroke by influencing a range of endpoints. However, further experiments are needed to reach any firm conclusion. 267 Study to evaluate correlation between experimentally induced numerous inflammatory models seizure and biochemical guidelines in rats Medhi B, Rao R S, Khanduja K L1, Pandhi P Division of Pharmacology and Biophysics1, Postgraduate Institute of Medical Education and Study, Chandigarh, India. Background: Oxidative stress has been implicated in the pathogenesis of various conditions including epilepsy, inflammatory bowel disease and rheumatoid arthritis (RA). Aim of the study was to produce various inflammatory models and seizure and to understand the effect of different medicines (thalidomide, etoricoxib) on seizure and to find out the correlation with antioxidant guidelines. Materials and Methods: Total of 54 male rats was included in the study. Rats were divided into 3 groups of acetic acid colitis, adjuvant arthritis, and cotton wool granuloma. Each group experienced 3 subgroups of control, model and treatment. Thalidomide was used as treatment in colitis and arthritis group while etoricoxib was used in cotton wool granuloma group. At the end of three days in colitis, seventeen days in arthritis and seven days in cotton wool granuloma group a subconvulsive dose of pentylenetetrazole (PTZ) (40mg/kg i.p.) was injected intraperitoneally to note seizure onset and seizure score. Presence of swelling was confirmed by morphology and histology. Plasma and mind biochemical guidelines like Malondialdehyde 7,8-Dihydroxyflavone (MDA), Superoxide dismutase (SOD), Glutathione peroxidase (GPx) were estimated. Results: The models of colitis and arthritis were effectively produced as evidenced by morphology scores ( 0.05). However, Sodium Valproate treated individuals who received Olanzapine in third week experienced 15.3% fall while individuals on Olanzapine who received Sodium Valproate experienced a 23.7% fall in the YMRS score. Patients who achieved serum valproic acid level of 100dunal and l-dopa in the inhibition of haloperidol-induced catalepsy in mice Gupta Girdhari Lal, Rana AC B. N. College of Pharmacy, Udaipur -313 002 (Rajasthan) India. The possible synergism between WS and dopamine precursor L-dopa to inhibit haloperidol-induced catalepsy was investigated by using standard bar test in mice. The effect of WS (20-200 mg/ kg, oral), L-dopa (20-200 mg/kg, oral) plus carbidopa (2-20.Still prospective work is going on to evaluate the part of 5-HT3 antagonist with this field. 316 Homocysteine levels in neurological disorders in Indian population Sharma S, Prakash A, Kushwaha S Institute of Human being Behavior and Allied Sciences, Delhi, India. Hyperhomocysteinemia is an important risk element for stroke and other neurological disorders and there is paucity of data in Indian populace. of hippocampal cytoarchitecture and improved apoptosis and the ER alpha and beta immunoreactive (ir) neurons were significantly (in oxygen glucose deprivation-pc-12 cell model of cerebral stroke Singh G1, Siddiqui MA1, Kashyap MP1, Pant KK2, Gupta YK3, Pant Abdominal1 1Indian Institute of Toxicology Study, Lucknow; 2CSM Medical University or college, Lucknow; 3All India Institute of Medical Sciences, New Delhi, India. Oxidative stress mediated neuronal injury is well recorded in ischemic cerebral stroke. The numbers of antioxidants have been looked at for his or her neuroprotective potential and is one of them. However, the mechanisms involved in restorative treatment of in cerebral stroke are yet to explore. Therefore, attempts were initiated to identify the points of restorative interventions of using oxygen-glucose deprivation (OGD)-Personal computer-12 cell model of ischemic stroke. The model was created by exposing the Personal computer-12 cells to OGD insult for 6 h following a re-oxygenation of 24 h under normoxia condition in glucose comprising medium. Biologically safe concentrations (25, 50, 100 g/ml) of recognized through MTT and LDH assays were used. Three treatment schedules were designed viz., pretreatment group (treatment for 24 h prior to OGD insult), post treatment group (treatment for 24 h after OGD insult) and whole treatment group (treatment starting from 24 h prior to OGD insult and continued till the completion of re-oxygenation period). Parallel units without OGD insult, OGD insult without treatment also run under identical conditions and served as basal and OGD settings respectively. Following respective treatments, cells were analyzed to study the mediated 7,8-Dihydroxyflavone repair of oxidative stress induced alterations viz., reactive oxygenation varieties (ROS), nitric oxide (NO), lipid peroxidation (LPO), glutathione content material (GSH), membrane potential, dopamine receptor (DA-D2) and prostaglandin E2 (PGE2). In general, a dose dependent significant recovery in the levels of PGE2, GSH and NO could be recorded in all three treatment organizations, however, pre- and post-treatment organizations were found to be most effective on ROS and DA-D2 repair. No significant response could be recognized for LPO and membrane potential. The initial getting suggests the restorative treatment of in LIMK2 cerebral stroke by influencing a range of endpoints. However, further experiments are needed to reach any firm conclusion. 267 Study to evaluate correlation between experimentally induced numerous inflammatory models seizure and biochemical guidelines in rats Medhi B, Rao R S, Khanduja K L1, Pandhi P Division of Pharmacology and Biophysics1, Postgraduate Institute of Medical Education and Study, Chandigarh, India. Background: Oxidative stress has been implicated in the pathogenesis of various conditions including epilepsy, inflammatory bowel disease and rheumatoid arthritis (RA). Aim of the study was to produce various inflammatory models and seizure and to understand the effect of different medicines (thalidomide, etoricoxib) on seizure and to find out the correlation with antioxidant guidelines. Materials and Methods: Total of 54 male rats was included in the study. Rats were divided into 3 groups of acetic acid colitis, adjuvant arthritis, and cotton wool granuloma. Each group experienced 3 subgroups of control, model and treatment. Thalidomide was used as treatment in colitis and arthritis group while etoricoxib was used in cotton wool granuloma group. At the end of three days in colitis, seventeen days in arthritis and seven days in cotton wool granuloma group a subconvulsive dose of pentylenetetrazole (PTZ) (40mg/kg i.p.) was injected intraperitoneally to note seizure onset and seizure score. Presence of swelling was confirmed by morphology and histology. Plasma and mind biochemical guidelines like Malondialdehyde (MDA), Superoxide dismutase (SOD), Glutathione peroxidase (GPx) were estimated. Results: The models of colitis and arthritis were effectively produced as evidenced by morphology scores ( 0.05). However, Sodium Valproate treated individuals who received Olanzapine in third week experienced 15.3% fall while individuals on Olanzapine who received Sodium Valproate experienced a 23.7% fall in the YMRS score. Patients who achieved serum valproic acid level of 100dunal and l-dopa in the inhibition of haloperidol-induced catalepsy in mice Gupta Girdhari Lal, Rana AC B. N. College of Pharmacy, Udaipur -313 002 (Rajasthan) India. The possible synergism between WS.